Further studies are needed to determine the clinical implications of REG1О± and other markers. With the best cut-off value, the sensitivity and specificity of REG1О± to differentiate normal and pancreatic cancer were 82.6 and 81.8%, compared with 69.6 and 100% for CA19-9.Д¬ONCLUSIONS: We have shown that pancreatic juice is a good source of pancreatic cancer tumor markers. ELISA showed that the serum level of REG1О± was significantly higher in patients with pancreatic cancer than it was in the normal controls (P = 0.023). Among the identified proteins, we confirmed the expressions of BIG2, PRDX6, and REG1О± in pancreatic cancer tissue using immunohistochemistry. After excluding spots that were also expressed in chronic pancreatitis, 26 protein spots that were up-regulated or only expressed in pancreatic cancer samples were identified. RESULTS: Thirty-five protein spots were up-regulated twofold in pancreatic cancer compared with the levels in the normal controls, and 85 protein spots were present in pancreatic cancer samples but not in normal controls. HCO 3-determines the pH of bodily fluids as a major buffer system that guards against toxic pH fluctuations (116). When stimulated, the pancreas secretes alkaline pancreatic juice containing copious amounts of bicarbonate (HCO 3-) (23, 74).HCO 3-plays essential roles in the digestive system. Pancreatic juices consisting of three pooled samples for normal control, chronic pancreatitis, and pancreatic cancer patients were compared using two-dimensional gel electrophoresis, and the proteins were subsequently identified using MALDI-TOF-MS. The human exocrine pancreas secretes 1-2 liters of pancreatic juice per day. METHODS: Secretin was injected intravenously and pancreatic juice was collected via selective cannulation of the pancreatic duct during endoscopic retrograde cholangiopancreatography. Innervation appears to play a critical permissive role in the effect of gastrointestinal regulatory peptides on the exocrine pancreas.Authors Jeong Youp Park Sun-A Kim Joo Won Chung Seungmin Bang Seung Woo Park Young-Ki Paik Si Young Song Citation JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, Vol.137(8) : 1229-1238, 2011 Journal Title JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY ISSN 0171-5216 Issue Date 2011 MeSH AdultВ В AgedВ В Biomarkers, Tumor/analysis*В В Biomarkers, Tumor/bloodВ В Chi-Square DistributionВ В Cholangiopancreatography, Endoscopic RetrogradeВ В Electrophoresis, Gel, Two-DimensionalВ В Enzyme-Linked Immunosorbent AssayВ В FemaleВ В Guanine Nucleotide Exchange Factors/analysisВ В HumansВ В ImmunohistochemistryВ В Lithostathine/analysis*В В Lithostathine/bloodВ В MaleВ В Middle AgedВ В Pancreatic Juice/chemistry*В В Pancreatic Neoplasms/chemistry*В В Pancreatitis/metabolismВ В Peroxiredoxin VI/analysisВ В Sensitivity and SpecificityВ В Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Keywords Pancreatic cancerВ В Pancreatic juiceВ В Biomarker Abstract INTRODUCTION: Protein profiles of endoscopically collected pancreatic juice from normal, chronic pancreatitis patients and pancreatic cancer patients were compared to identify diagnostic biomarkers of pancreatic cancer. Some gastrointestinal regulatory peptides affect the pancreas indirectlyДЇrom the site of their release, i.e., the proximal duodenum in preruminant calves. The importance of the vagal nerves, gastrointestinal regulatory peptides, and duodenal contents in the regulation of pancreatic secretion in youngДЄnd adult cattle is discussed. Insulo-acinar axis may be important in this respect. Manipulation of the diet effectively changed daily exocrine secretion, and it is hypothesized that the

Pancreatic secretion was relatively stable and essentially did not respond to feeding. Pancreatic oscillations developed with the maturation of the gastrointestinal tract. Plain and chromic catgut, polyglactin 910, polyglycolic acid, polydioxanone, polypropylene, and silk sutures were incubated in pancreatic juice and. With the duodenal migrating motor complex were observed in calves from 2 nd-3 rd day of life. Synchronized periodic oscillations of pancreatic secretion in phase The secretion of pancreatic juice was studied in cattle from the first days of life to the start of